Clinical utility of fractionating erythrocytes into "Percoll" density gradients

Adv Exp Med Biol. 1991:307:227-38. doi: 10.1007/978-1-4684-5985-2_21.

Abstract

Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anemia, Hemolytic / blood
  • Cell Fractionation / methods*
  • Centrifugation, Density Gradient*
  • Child
  • Clinical Enzyme Tests
  • Drug Compounding
  • Erythrocyte Aging
  • Erythrocytes / enzymology*
  • Female
  • Fructose-Bisphosphate Aldolase / blood
  • Glucosephosphate Dehydrogenase / blood*
  • Glucosephosphate Dehydrogenase Deficiency / blood*
  • Heterozygote
  • Hexokinase / blood
  • Humans
  • Male
  • Phosphogluconate Dehydrogenase / blood*
  • Phytic Acid / administration & dosage
  • Povidone
  • Pyruvate Kinase / blood
  • Pyruvate Kinase / deficiency*
  • Reticulocytes / enzymology
  • Silicon Dioxide
  • Thalassemia / blood*

Substances

  • Percoll
  • Silicon Dioxide
  • Phytic Acid
  • Phosphogluconate Dehydrogenase
  • Glucosephosphate Dehydrogenase
  • Hexokinase
  • Pyruvate Kinase
  • Fructose-Bisphosphate Aldolase
  • Povidone