Abstract
Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Anemia, Hemolytic / blood
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Cell Fractionation / methods*
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Centrifugation, Density Gradient*
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Child
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Clinical Enzyme Tests
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Drug Compounding
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Erythrocyte Aging
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Erythrocytes / enzymology*
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Female
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Fructose-Bisphosphate Aldolase / blood
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Glucosephosphate Dehydrogenase / blood*
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Glucosephosphate Dehydrogenase Deficiency / blood*
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Heterozygote
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Hexokinase / blood
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Humans
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Male
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Phosphogluconate Dehydrogenase / blood*
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Phytic Acid / administration & dosage
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Povidone
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Pyruvate Kinase / blood
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Pyruvate Kinase / deficiency*
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Reticulocytes / enzymology
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Silicon Dioxide
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Thalassemia / blood*
Substances
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Percoll
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Silicon Dioxide
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Phytic Acid
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Phosphogluconate Dehydrogenase
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Glucosephosphate Dehydrogenase
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Hexokinase
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Pyruvate Kinase
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Fructose-Bisphosphate Aldolase
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Povidone