Abstract
Brucellosis is a severe systemic disease in humans. We describe a new 16S rRNA-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of Brucella and that can be applied directly to positive blood cultures.
MeSH terms
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Bacteremia / diagnosis
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Bacteremia / microbiology
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Bacterial Typing Techniques
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Bacteriological Techniques / methods*
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Base Sequence
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Brucella / classification
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Brucella / genetics*
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Brucella / isolation & purification*
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Brucella / pathogenicity
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Brucellosis / diagnosis
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Brucellosis / microbiology
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DNA Probes / genetics
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DNA, Bacterial / genetics
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Humans
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In Situ Hybridization, Fluorescence / methods*
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Species Specificity
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Virulence / genetics
Substances
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DNA Probes
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DNA, Bacterial