Polymorphism of the cell wall-anchoring domain of the autolysin-adhesin AtlE and its relationship to sequence type, as revealed by multilocus sequence typing of invasive and commensal Staphylococcus epidermidis strains

J Clin Microbiol. 2006 May;44(5):1839-43. doi: 10.1128/JCM.44.5.1839-1843.2006.

Abstract

We sequenced the adhesin-cell wall-anchoring domain of the atlE gene of 49 invasive and commensal Staphylococcus epidermidis strains. We identified 22 alleles, which could be separated into two main groups: group 1 (alleles 1 and 6 to 16, 32/49 strains) and group 2 (alleles 2 to 5 and 17 to 22, 17/49 strains). Allele 1 (the type strain sequence) was by far the most prevalent (21 of 49 strains). Multilocus sequence typing showed a clear relationship between the atlE allele and the sequence type (ST), with the "nosocomial" ST27 clone and closely related STs expressing group 1 alleles.

MeSH terms

  • Adhesins, Bacterial / genetics*
  • Alleles
  • Amino Acid Sequence
  • Bacterial Typing Techniques
  • Base Sequence
  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Humans
  • Molecular Sequence Data
  • N-Acetylmuramoyl-L-alanine Amidase / genetics*
  • Phylogeny
  • Polymorphism, Genetic
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Staphylococcal Infections / microbiology
  • Staphylococcus epidermidis / classification
  • Staphylococcus epidermidis / genetics*
  • Staphylococcus epidermidis / isolation & purification
  • Staphylococcus epidermidis / pathogenicity
  • Virulence / genetics

Substances

  • Adhesins, Bacterial
  • DNA, Bacterial
  • N-Acetylmuramoyl-L-alanine Amidase

Associated data

  • GENBANK/AJ887965
  • GENBANK/AJ887966
  • GENBANK/AJ887967
  • GENBANK/AJ887968
  • GENBANK/AJ887969
  • GENBANK/AJ887970
  • GENBANK/AJ887971
  • GENBANK/AJ887972
  • GENBANK/AJ887973
  • GENBANK/AJ887974
  • GENBANK/AJ887975
  • GENBANK/AJ887976
  • GENBANK/AJ887977
  • GENBANK/AJ887978
  • GENBANK/AJ887979
  • GENBANK/AJ887980
  • GENBANK/AJ887981
  • GENBANK/AJ887982
  • GENBANK/AJ887983
  • GENBANK/AJ887984
  • GENBANK/AJ887985
  • GENBANK/AJ887986