Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers

Appl Environ Microbiol. 2006 May;72(5):3766-70. doi: 10.1128/AEM.72.5.3766-3770.2006.

Abstract

Fecal culture for Escherichia coli O157:H7 was compared to rectoanal mucosal swab (RAMS) culture in dairy heifers over a 1-year period. RAMS enrichment culture was as sensitive as fecal culture using immunomagnetic separation (IMS) (P = 0.98, as determined by a chi-square test). RAMS culture is less costly than fecal IMS culture and can yield quantitative data.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anal Canal / microbiology*
  • Animals
  • Bacteriological Techniques
  • Cattle / microbiology*
  • Culture Media
  • Dairying*
  • Escherichia coli O157 / isolation & purification*
  • Feces / microbiology*
  • Female
  • Mucous Membrane / microbiology
  • Rectum / microbiology*
  • Specimen Handling / methods

Substances

  • Culture Media