Abstract
An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Drug Resistance, Bacterial / genetics*
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Escherichia coli / drug effects*
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Escherichia coli / genetics
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Escherichia coli / pathogenicity*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Gene Expression Profiling
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Humans
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Oligonucleotide Array Sequence Analysis / methods*
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Virulence / genetics
Substances
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Escherichia coli Proteins