Abstract
To study hormone-regulated inflorescence development, we established the in vitro regeneration system of Arabidopsis inflorescences in the presence of cytokinin and auxin. Media containing a combination of thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) were used to induce callus formation. Higher frequencies of calli were obtained by using the inflorescence stems as explants. After transferring the calli to media containing a combination of zeatin and indole-3-acetic acid (IAA), the inflorescences were induced from the calli. The morphology of regenerated inflorescences was similar to that of inflorescences in plants; however, flowers of regenerated inflorescences often lacked a few floral organs. Furthermore, TFL1, a gene involved in floral transition in Arabidopsis, was activated during the inflorescence induction. Our results suggest that the TFL1 gene plays an important role in hormone-regulated inflorescence formation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2,4-Dichlorophenoxyacetic Acid / pharmacology
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Arabidopsis / drug effects
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Arabidopsis / growth & development
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Arabidopsis / physiology*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism
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Cytokinins / pharmacology
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Flowers / drug effects
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Flowers / growth & development
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Flowers / physiology*
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Gene Expression Regulation, Plant / drug effects
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Indoleacetic Acids / pharmacology
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Phenylurea Compounds / pharmacology
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Plant Growth Regulators / pharmacology*
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Plant Stems / drug effects
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Plant Stems / growth & development
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Plant Stems / physiology
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Promoter Regions, Genetic
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Regeneration
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Thiadiazoles / pharmacology
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Zeatin / pharmacology
Substances
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Arabidopsis Proteins
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Chromosomal Proteins, Non-Histone
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Cytokinins
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Indoleacetic Acids
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Phenylurea Compounds
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Plant Growth Regulators
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TFL1 protein, Arabidopsis
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TFL2 protein, Arabidopsis
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Thiadiazoles
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thidiazuron
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2,4-Dichlorophenoxyacetic Acid
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indoleacetic acid
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Zeatin