Influence of donor C3 allotype on late renal-transplantation outcome

N Engl J Med. 2006 May 11;354(19):2014-23. doi: 10.1056/NEJMoa052825.

Abstract

Background: The complement system has a critical role in both the innate and the adaptive immune responses. In humans, C3 exists as two main allotypes, F (fast) and S (slow), which are known to affect the incidence of inflammatory disease. We conducted a study to address the influence of these alleles on late renal-graft outcome.

Methods: We determined the C3 allotypes of 662 pairs of adult kidney donors and recipients from 1993 through 2002 and then related C3F/S polymorphism status to demographic and clinical outcome data. The median length of follow-up was 3.3 years.

Results: Analysis of 513 pairs of white donors and recipients identified 113 C3S/S recipients of a C3S/F or a C3F/F kidney and 179 C3S/S recipients of a C3S/S kidney. Graft survival was significantly better with a C3F/F or C3F/S donor allotype than a C3S/S allotype (P=0.05). The hazard ratio for graft loss of C3S/S kidneys, as compared with C3F/F or C3F/S kidneys, was 2.21 (95 percent confidence interval, 1.04 to 4.72; P=0.04). The graft function of C3F/F or C3F/S donor kidneys was significantly better than that of C3S/S donor kidneys (P<0.001). The effect of the C3F allele was specific to recipients who did not themselves possess this allele. Multivariate analysis excluded effects of other factors known to influence graft outcome.

Conclusions: Expression of C3 alleles by donor renal cells appears to have a differential effect on late graft outcome. Among white C3S/S recipients, receipt of a C3F/F or C3F/S donor kidney, rather than a C3S/S donor kidney, is associated with a significantly better long-term outcome. These findings suggest that the two alleles have functional differences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alleles
  • Complement C3 / chemistry
  • Complement C3 / genetics*
  • Female
  • Follow-Up Studies
  • Histocompatibility Testing
  • Humans
  • Kidney Transplantation / immunology*
  • Male
  • Multivariate Analysis
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Protein Conformation
  • Sequence Analysis, DNA
  • Tissue Donors*
  • Transplantation, Homologous
  • Treatment Outcome
  • White People / genetics

Substances

  • Complement C3