[A cytological, immunophenotypical and cytogenetical study of 136 consecutive cases of B-cell chronic lymphoid hemopathies]

Pathol Biol (Paris). 2007 Feb;55(1):59-72. doi: 10.1016/j.patbio.2006.04.006. Epub 2006 May 11.
[Article in French]

Abstract

A cytological, immunophenotypical and cytogenetical study of 136 chronic B-cell proliferations (93 CLL, 43 B-cell lymphomas) was led in order to precise diagnosis and to characterize and appreciate chromosomal rearrangements. In this series, mainly selected on blood lymphocytosis criteria, B-CLL were twice more frequent than small B-cell lymphomas. Probes used revealed cryptic abnormalities, which remained unknown by conventional cytogenetics (CC). The frequency of clonal abnormalities (CC and FISH) was 74.8% for this series, with 74.4% for lymphomas and 75.3% for CLL, mainly of Binet stage A (69 A, 13 B, 1 C, 10 unspecified). Proportion was 88.4% in A stages and 84.6% in B stages. In CLL, 13q14 cryptic deletions and translocations were widely majority, 14q32 translocations and trisomy 12 being predominant in lymphoma series. Interphase FISH study of non-clonal metaphasic abnormalities with locus-specific probes often revealed unrecognised clones.

Publication types

  • English Abstract

MeSH terms

  • Aneuploidy
  • Chromosome Aberrations*
  • Chromosomes, Human / genetics*
  • Chromosomes, Human / ultrastructure
  • Chromosomes, Human, Pair 13 / genetics
  • Chromosomes, Human, Pair 13 / ultrastructure
  • Clone Cells / pathology
  • Cohort Studies
  • Female
  • Humans
  • Immunophenotyping
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Leukemia, Lymphocytic, Chronic, B-Cell / pathology
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / pathology
  • Lymphoproliferative Disorders / genetics
  • Male
  • Neoplasm Staging
  • Sequence Deletion