Effect of PD I administration on dopamine receptors mRNAs expression in the lesioned striatum of PD rat model

J Huazhong Univ Sci Technolog Med Sci. 2005;25(6):645-7. doi: 10.1007/BF02896159.

Abstract

To study the effect of PD I administration on dopamine receptors (DR1, DR2) mRNAs expression in the lesioned striatum of the PD rat model and confirm if PD I has the effect of dopamine receptor agonist. The PD rats with unilateral 6-hydroxydopamine lesioned were administrated with PD I, L-dopa methyl/benserazide, L-dopa methyl /benserazide/ PD I, normal saline respectively for 4 weeks and their behavioral changes were observed. Then the rats were sacrificed and RT-PCR technique was used to detect changes of dopamine receptors (DR1, DR2) mRNAs expression in the ipsilateral striatum 1 day after the last treatment. The results showed that treatment with PD I plus L-dopa resulted in a stable contralateral rotation behavior; treatment with L-dopa resulted in a progressively increased contralateral rotation behavior. Rotation behavior induced by anhydromorphine decreased with PD I or PD I plus L-dopa treatment. Treatment With L-dopa or PD I plus L-dopa, up-regulation of DR1 mRNA and down-regulation of DR2 mRNA were observed in the ipsilateral striatum which were more obvious than that treated with PD I or vehicle (P < 0.05). It was concluded that long-term treatment with PD I could alleviate the behavior of PD rats. PD I had no apparent effect on the dopamine receptors (DR1, DR2) mRNAs expression in the ipsilateral striatum and the PD I has no agonist effect on dopamine receptors.

MeSH terms

  • Animals
  • Corpus Striatum / metabolism
  • Dopamine Agonists / therapeutic use
  • Drugs, Chinese Herbal / therapeutic use*
  • Female
  • Parkinsonian Disorders / drug therapy*
  • Parkinsonian Disorders / metabolism
  • Phytotherapy*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine D1 / biosynthesis*
  • Receptors, Dopamine D1 / genetics
  • Receptors, Dopamine D2 / biosynthesis*
  • Receptors, Dopamine D2 / genetics

Substances

  • Dopamine Agonists
  • Drugs, Chinese Herbal
  • RNA, Messenger
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2