Abstract
Beta-Xylosides stimulate 2- to 6-fold the synthesis of glycosaminoglycans by three types of nonconnective tissue cells (RG-C6, NB41A, and rat hepatoma cells, and normal and simian virus 40 (SV40)-transformed normal human skin fibroblasts. The effect, which is specific for the anomeric linkage and the glycone, is observed in the presence and absence of puromycin. Beta-Xylosides may substitute for xylosylated core protein as initiators of synthesis of chondroitin sulfate chains. No stimulation of synthesis of heparan sulfate was observed. With the use of a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside, it was demonstrated that the free chondroitin sulfate chains secreted into the medium bear the xyloside at the reducing end, and have an average molecular weight of 16,500.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carcinoma, Hepatocellular / metabolism
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Cells, Cultured
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Chondroitin / biosynthesis*
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Chromatography, Gel
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Dermatan Sulfate / analysis
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Fibroblasts / metabolism
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Galactose / pharmacology
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Glycosaminoglycans / biosynthesis*
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Glycosides / pharmacology
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Heparitin Sulfate / analysis
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Humans
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Hyaluronic Acid / analysis
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Hymecromone / pharmacology
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Liver Neoplasms
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Mice
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Neuroblastoma / metabolism
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Neuroglia / metabolism
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Nitrophenols / pharmacology
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Rats
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Simian virus 40
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Skin
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Spectrometry, Fluorescence
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Sulfur Radioisotopes
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Sulfuric Acids / biosynthesis
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Tritium
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Xylose / pharmacology*
Substances
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Glycosaminoglycans
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Glycosides
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Nitrophenols
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Sulfur Radioisotopes
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Sulfuric Acids
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Tritium
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Dermatan Sulfate
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Hymecromone
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Hyaluronic Acid
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Chondroitin
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Heparitin Sulfate
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Xylose
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Galactose