Abstract
We report the first isolation and characterization of the canine NKX2-5 gene. This canine homologue has high homology in genomic structure and functional domains to other NKX2-5 across a number of different species. Given the critical role of NKX2-5 in cardiac morphogenesis as seen in human and mouse studies of congenital heart defects, the availability of the canine NKX2-5 provides a good starting point for identifying mutations that may be responsible for certain forms of canine congenital heart defects.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence / genetics
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DNA Primers / chemistry
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Dogs / genetics*
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Female
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Gene Order
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Genes, Homeobox / genetics*
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Homeobox Protein Nkx-2.5
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / genetics*
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Molecular Sequence Data
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Mutation / genetics
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Polymerase Chain Reaction
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics*
Substances
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DNA Primers
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Homeobox Protein Nkx-2.5
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Homeodomain Proteins
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NKX2-5 protein, human
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Transcription Factors
Associated data
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GENBANK/DQ220791
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GENBANK/DQ350454