Location and abundance of poly (A) sequences in Sendai virus messenger RNA molecules

J Gen Virol. 1975 May;27(2):247-50. doi: 10.1099/0022-1317-27-2-247.

Abstract

Adenine-rich sequences from 18S Sendai virus messenger RNA species were 99% adenylate, 3'-OH terminal, and were present in at least 50% of the RNA molecules. Intact virus messenger RNA molecules were resistant to exonucleolytic attack by polynucleotide phosphorylase, suggesting that their 3'-termini are masked.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenine / analysis
  • Adenine Nucleotides
  • Animals
  • Base Sequence
  • Centrifugation, Density Gradient
  • Chick Embryo
  • Culture Techniques
  • Dactinomycin / pharmacology
  • Lung
  • Micrococcus / enzymology
  • Parainfluenza Virus 1, Human / analysis*
  • Parainfluenza Virus 1, Human / metabolism
  • Polynucleotides / analysis*
  • Polyribonucleotide Nucleotidyltransferase / metabolism
  • RNA, Messenger / analysis*
  • RNA, Messenger / metabolism
  • RNA, Viral / analysis*
  • RNA, Viral / metabolism
  • Ribonucleases / metabolism

Substances

  • Adenine Nucleotides
  • Polynucleotides
  • RNA, Messenger
  • RNA, Viral
  • Dactinomycin
  • Polyribonucleotide Nucleotidyltransferase
  • Ribonucleases
  • Adenine