Elevated mutation rates in the germline of Polkappa mutant male mice

Karen L-A Burr; Susana Velasco-Miguel; Venkata S Duvvuri; Lisa D McDaniel; Errol C Friedberg; Yuri E Dubrova

doi:10.1016/j.dnarep.2006.04.003

Elevated mutation rates in the germline of Polkappa mutant male mice

DNA Repair (Amst). 2006 Jul 13;5(7):860-2. doi: 10.1016/j.dnarep.2006.04.003. Epub 2006 May 30.

Authors

Karen L-A Burr¹, Susana Velasco-Miguel, Venkata S Duvvuri, Lisa D McDaniel, Errol C Friedberg, Yuri E Dubrova

Affiliation

¹ Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.

PMID: 16731053
DOI: 10.1016/j.dnarep.2006.04.003

Abstract

Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of DNA polymerase kappa (Polkappa(-/-)) deficient mice. The spontaneous mutation rate in homozygous Polkappa(-/-) males was significantly higher than in isogenic wild-type mice (Polkappa(+/+)), but the ESTR mutation spectrum in Polkappa(-/-) animals did not differ from that in Polkappa(+/+) males. We suggest that compromised translesion synthesis in Polkappa(-/-) mice may result in replication fork pausing which, in turn, may affect ESTR mutation rate.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Damage
DNA Repair / genetics
DNA-Directed DNA Polymerase / deficiency
DNA-Directed DNA Polymerase / genetics*
Germ-Line Mutation*
Male
Mice
Mice, Inbred BALB C
Mice, Knockout
Minisatellite Repeats

Substances

DNA-Directed DNA Polymerase
Polk protein, mouse