Soma sizes of embryonic male and female tyrosine hydroxylase-immunoreactive neurons were measured in 3 diencephalic regions in situ and in diencephalic dissociated cell cultures. Male neurons were about 30% larger than female neurons both in vitro and in situ. Treatment of cultures with sex steroids did not affect the sex differences. It is concluded that sexual differentiation of dopaminergic neurons may be under primary genetic control.