Use of the housekeeping genes, gdh (zwf) and gki, in multilocus sequence typing to differentiate Streptococcus pneumoniae from Streptococcus mitis and Streptococcus oralis

Diagn Microbiol Infect Dis. 2006 Nov;56(3):321-4. doi: 10.1016/j.diagmicrobio.2006.04.013. Epub 2006 Jun 12.

Abstract

Polymerase chain reaction and sequencing of the housekeeping genes, gdh (zwf) and gki, based on the primers and alleles from multilocus sequence typing can be used to delineate and support the identity of clinical isolates of Streptococcus pneumoniae and differentiate from the closely related Streptococcus mitis and Streptococcus oralis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Genes, Bacterial*
  • Phylogeny
  • Streptococcus mitis / genetics
  • Streptococcus mitis / isolation & purification*
  • Streptococcus oralis / genetics
  • Streptococcus oralis / isolation & purification*
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / isolation & purification*
  • Sugar Alcohol Dehydrogenases / genetics*
  • Sugar Alcohol Dehydrogenases / isolation & purification

Substances

  • Sugar Alcohol Dehydrogenases
  • galactitol 2-dehydrogenase