Immunogenicity of cytopathic and noncytopathic viral vectors

J Virol. 2006 Jul;80(13):6259-66. doi: 10.1128/JVI.00084-06.

Abstract

The impact of cytolytic versus noncytolytic viral infections on host responses is not well understood, due to limitations of the systems that have been used to address this issue. Using paired cytopathic and noncytopathic rabies viruses that differ by only two amino acids, we investigated several fundamental aspects of the immune response to these viral vectors. Greater cytopathic capacity translated into a greater degree of cross-priming to CD8(+) T cells (T(CD8)(+)) and more-robust short-term humoral and cellular responses. However, long-term responses to the two viruses were similar, suggesting that direct priming drives the bulk of the T(CD8)(+) antirabies response and that enhanced acute responses associated with greater virally mediated cellular destruction were balanced by other factors, such as prolonged antigen expression associated with noncytopathic virus. Such compensatory mechanisms may be in place to ensure comparable immunologic memories to various pathogens.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibody Formation / genetics
  • Antibody Formation / immunology
  • Antigens, Viral / genetics
  • Antigens, Viral / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Cytopathogenic Effect, Viral / genetics
  • Cytopathogenic Effect, Viral / immunology
  • Female
  • Genetic Vectors / genetics
  • Genetic Vectors / immunology
  • Immunity, Cellular / genetics
  • Immunity, Cellular / immunology
  • Immunologic Memory / genetics
  • Immunologic Memory / immunology
  • Mice
  • Rabies / genetics
  • Rabies / immunology*
  • Rabies / prevention & control
  • Rabies Vaccines / genetics
  • Rabies Vaccines / immunology*
  • Rabies virus / genetics
  • Rabies virus / immunology*
  • Time Factors

Substances

  • Antigens, Viral
  • Rabies Vaccines