GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis

Rasmus Larsen; Tomas G Kloosterman; Jan Kok; Oscar P Kuipers

doi:10.1128/JB.00025-06

GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis

J Bacteriol. 2006 Jul;188(13):4978-82. doi: 10.1128/JB.00025-06.

Authors

Rasmus Larsen¹, Tomas G Kloosterman, Jan Kok, Oscar P Kuipers

Affiliation

¹ Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.

Abstract

We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TNACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.

MeSH terms

Bacterial Proteins / genetics*
Base Sequence
Gene Expression Regulation, Bacterial
Glutamine
Lactococcus lactis / genetics*
Lactococcus lactis / growth & development
Lactococcus lactis / metabolism*
Molecular Sequence Data
Nitrogen / metabolism*
Operon / genetics
Quaternary Ammonium Compounds
Trans-Activators / genetics*

Substances

Bacterial Proteins
Quaternary Ammonium Compounds
Trans-Activators
Glutamine
Nitrogen