Abstract
This chapter discusses experimental methods and protocols for the purification and preliminary characterization of DNA polymerases that are specialized for the replicative bypass (translesion DNA synthesis) of base or other types of DNA damage that typically arrest high-fidelity DNA synthesis, with particular emphasis on DNA polymerase kappa (Polkappa from mouse cells). It also describes some of the methods employed in the evaluation of mouse strains defective in genes that encode these enzymes.
MeSH terms
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Animals
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B-Lymphocytes / immunology
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B-Lymphocytes / physiology
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Cells, Cultured
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DNA / chemistry
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DNA / metabolism
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DNA / radiation effects
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DNA Damage*
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DNA Replication*
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / isolation & purification
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DNA-Directed DNA Polymerase / metabolism*
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Mice
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Models, Animal
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Ultraviolet Rays
Substances
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Recombinant Proteins
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DNA
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DNA-Directed DNA Polymerase