[Cloning of A73 gene and its coding sequence analysis]

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jun;26(6):826-7, 830.
[Article in Chinese]

Abstract

Objective: To clone A73 gene of Epstein-Barr virus (EBV) and examine the variation of its coding sequence.

Methods: A73 coding sequence (CDS) amplified from 7 patients in nasophryngeal carcinoma (NPC) biopsies by RT-PCR was cloned into pGEM-T-Easy vector to construct the recombinant plasmid, which was subjected to sequence analysis in Gen Bank database using Blast software.

Results: A73 gene from nasophryngeal carcinoma was successfully cloned into pGEM-T Easy vector. A locus with conversion of A-->C in A73 was found at CDS 45 nt, located in the exonVB157154 nt, which did not result in an amino acid replacement, and the variation frequency was 7/7.

Conclusion: There is a point mutation in A73 CDS of EBV isolated from NPC tissue, which might produce NPC-associated polymorphism with possible involvement in the composition of some subtype of EB virus.

Publication types

  • English Abstract

MeSH terms

  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Molecular Sequence Data
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / virology
  • Open Reading Frames / genetics
  • Point Mutation
  • Sequence Analysis, DNA
  • Viral Proteins / genetics*

Substances

  • DNA, Complementary
  • Viral Proteins

Associated data

  • GENBANK/AJ251096