Immobilization of proteins on agarose beads, monitored in real time by bead injection spectroscopy

Analyst. 2006 Jul;131(7):799-808. doi: 10.1039/b603768b. Epub 2006 May 17.

Abstract

A novel approach to real-time monitoring of protein immobilization resulted in the surprising finding that current immobilization protocols are far from optimized.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flow Injection Analysis / methods
  • Microchemistry / methods*
  • Microspheres
  • Proteins / chemistry*
  • Sepharose
  • Spectrum Analysis / methods

Substances

  • Proteins
  • Sepharose