Abstract
The Marburg virus (MARV), an African filovirus closely related to the Ebola virus, causes a deadly hemorrhagic fever in humans, with up to 90% mortality. Currently, treatment of disease is only supportive, and no vaccines are available to prevent spread of MARV infections. In order to address this need, we have developed and characterized a novel recombinant vaccine that utilizes a single complex adenovirus-vectored vaccine (cAdVax) to overexpress a MARV glycoprotein (GP) fusion protein derived from the Musoke and Ci67 strains of MARV. Vaccination with the cAdVaxM(fus) vaccine led to efficient production of MARV-specific antibodies in both mice and guinea pigs. Significantly, guinea pigs vaccinated with at least 5 x 10(7) pfu of cAdVaxM(fus) vaccine were 100% protected against lethal challenges by the Musoke, Ci67 and Ravn strains of MARV, making it a vaccine with trivalent protective efficacy. Therefore, the cAdVaxM(fus) vaccine serves as a promising vaccine candidate to prevent and contain multi-strain infections by MARV.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenoviridae / genetics
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Adenoviridae / metabolism*
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Amino Acid Sequence
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Animals
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Antibodies, Viral / blood
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Antigens, Viral / biosynthesis*
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Antigens, Viral / genetics
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Cell Line
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Chlorocebus aethiops
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Dose-Response Relationship, Immunologic
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Ebola Vaccines / administration & dosage*
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Ebola Vaccines / genetics
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Genetic Therapy / methods
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Genetic Vectors / metabolism*
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Guinea Pigs
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Humans
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Injections, Intraperitoneal
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Injections, Subcutaneous
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Marburg Virus Disease / blood
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Marburg Virus Disease / immunology
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Marburg Virus Disease / prevention & control*
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Marburgvirus / immunology*
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Mice
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Molecular Sequence Data
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Sequence Alignment
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Vaccination*
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Vaccines, Synthetic / administration & dosage
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Viral Envelope Proteins / biosynthesis*
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Viral Envelope Proteins / genetics
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Viral Fusion Proteins / biosynthesis*
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Viral Fusion Proteins / genetics
Substances
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Antibodies, Viral
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Antigens, Viral
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Ebola Vaccines
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GP-protein, Marburg virus
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Recombinant Proteins
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Fusion Proteins