Lactobacillus cypricasei Lawson et al. 2001 is a later heterotypic synonym of Lactobacillus acidipiscis Tanasupawat et al. 2000

Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1681-1683. doi: 10.1099/ijs.0.64229-0.

Abstract

The applicability of a multilocus sequence analysis (MLSA)-based identification system for lactobacilli was evaluated. Two housekeeping genes that code for the phenylalanyl-tRNA synthase alpha-subunit (pheS) and RNA polymerase alpha-subunit (rpoA) were sequenced and analysed for members of the Lactobacillus salivarius species group. The type strains of Lactobacillus acidipiscis and Lactobacillus cypricasei were investigated further using a third gene that encodes the alpha-subunit of ATP synthase (atpA). The MLSA data revealed close relatedness between L. acidipiscis and L. cypricasei, with 99.8-100 % pheS, rpoA and atpA gene sequence similarities. Comparison of the 16S rRNA gene sequences of the type strains of the two species confirmed the close relatedness (99.8 % gene sequence similarity) between the two taxa. Similar phenotypes and high DNA-DNA binding values in the range of 84 to 97.5 % confirmed that L. acidipiscis and L. cypricasei are synonymous species. On the basis of the present study, it is proposed that Lactobacillus cypricasei is a later heterotypic synonym of Lactobacillus acidipiscis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Lactobacillus / classification*
  • Lactobacillus / genetics
  • Mitochondrial Proton-Translocating ATPases / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenylalanine-tRNA Ligase / genetics
  • Phylogeny
  • Protein Subunits / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Protein Subunits
  • RNA, Ribosomal, 16S
  • DNA-Directed RNA Polymerases
  • RNA polymerase alpha subunit
  • Mitochondrial Proton-Translocating ATPases
  • Phenylalanine-tRNA Ligase

Associated data

  • GENBANK/AM087679
  • GENBANK/AM087687
  • GENBANK/AM087708
  • GENBANK/AM087717
  • GENBANK/AM087721
  • GENBANK/AM087734
  • GENBANK/AM087737
  • GENBANK/AM087740
  • GENBANK/AM087746
  • GENBANK/AM087756
  • GENBANK/AM087760
  • GENBANK/AM087762
  • GENBANK/AM087784
  • GENBANK/AM087801
  • GENBANK/AM087831
  • GENBANK/AM087849
  • GENBANK/AM168423
  • GENBANK/AM168424
  • GENBANK/AM168425
  • GENBANK/AM168426
  • GENBANK/AM168427
  • GENBANK/AM168428
  • GENBANK/AM168429
  • GENBANK/AM168431
  • GENBANK/AM168432
  • GENBANK/AM168433