Solution NMR of membrane proteins: practice and challenges

Magn Reson Chem. 2006 Jul:44 Spec No:S24-40. doi: 10.1002/mrc.1816.

Abstract

This review focuses upon the application of solution NMR methods to multispan integral membrane proteins, particularly with respect to determination of global folds by this approach. Practical methods are described along with the special difficulties that confront the application of solution NMR to proteins that dwell in the netherworld of the lipid bilayer.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Amino Acid Sequence
  • Detergents / chemistry
  • Lipid Bilayers
  • Membrane Proteins / chemistry*
  • Membrane Proteins / classification
  • Micelles
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Folding
  • Solutions / chemistry
  • Solvents / chemistry*

Substances

  • Detergents
  • Lipid Bilayers
  • Membrane Proteins
  • Micelles
  • Solutions
  • Solvents