Investigating cis-acting regulatory variation using assays of relative allelic expression

Psychiatr Genet. 2006 Aug;16(4):173-7. doi: 10.1097/01.ypg.0000218612.35139.84.

Abstract

Assays of relative allelic expression offer a powerful means of exploring the influence of cis-acting regulatory variation. In this article, we provide a general assay protocol and discuss practical and theoretical considerations for the design and interpretation of relative allelic expression analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / genetics
  • DNA / isolation & purification
  • DNA Primers
  • Gene Expression Regulation*
  • Genetic Variation*
  • Humans
  • Models, Genetic
  • Polymorphism, Single Nucleotide
  • RNA / genetics
  • RNA / isolation & purification
  • Transcription, Genetic

Substances

  • DNA Primers
  • RNA
  • DNA