Pax-5 immunoexpression in various types of benign and malignant tumours: a high-throughput tissue microarray analysis

J Clin Pathol. 2007 Jun;60(6):709-14. doi: 10.1136/jcp.2006.039917. Epub 2006 Jul 12.

Abstract

Background: Pax-5 belongs to the Pax gene family transcription factors that play an important role in organogenesis and in B cell ontogeny. It is expressed in B cell non-Hodgkin's lymphoma (B-NHL), Hodgkin's lymphoma (HL) and neuroendocrine carcinomas. However, its expression in other tumour types is not fully explored.

Aims and methods: To determine Pax-5 expression in other tumour types, immunohistochemistry was performed on 3758 benign and malignant tumours using multiple tumour microarrays, as well as on whole sections.

Results: Pax-5 was expressed in 108/118 (91.5%) B-NHLs, in 60/70 (85.7%) HLs and in 0/7 T cell lymphomas. In addition, Pax-5 was seen in 24/34 (70.6%) Merkel cell carcinomas, 42/53 (79.2%) small cell carcinomas, 1/164 (0.6%) breast carcinomas, 2/204 (1%) endometrial adenocarcinomas and 1/452 (0.2%) urothelial carcinoma of the bladder.

Conclusion: Despite its expression in a small subset of malignancies of epithelial origin, Pax-5 is still a good and reliable immunomarker in diagnosing B-NHL, HL and neuroendocrine carcinomas.

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • Carcinoma, Merkel Cell / diagnosis
  • Carcinoma, Merkel Cell / metabolism
  • Carcinoma, Squamous Cell / diagnosis
  • Carcinoma, Squamous Cell / metabolism
  • Diagnosis, Differential
  • Hodgkin Disease / diagnosis
  • Hodgkin Disease / metabolism
  • Humans
  • Immunoenzyme Techniques
  • Lung Neoplasms / diagnosis
  • Lung Neoplasms / metabolism
  • Lymphoma, Non-Hodgkin / diagnosis
  • Lymphoma, Non-Hodgkin / metabolism
  • Neoplasm Proteins / metabolism
  • Neoplasms / diagnosis
  • Neoplasms / metabolism*
  • PAX5 Transcription Factor / metabolism*
  • Protein Array Analysis / methods

Substances

  • Biomarkers, Tumor
  • Neoplasm Proteins
  • PAX5 Transcription Factor
  • PAX5 protein, human