Abstract
During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae Infections / metabolism
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Adenoviridae Infections / virology
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Adenoviridae* / pathogenicity
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Adenoviridae* / physiology
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Binding Sites
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Biological Transport
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Capsid Proteins / physiology
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Cytoplasm / metabolism*
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Dependovirus / genetics*
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Dependovirus / pathogenicity
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Dependovirus / physiology
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Dyneins / metabolism*
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Genome, Viral
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Humans
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Microtubules* / metabolism
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Microtubules* / virology
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Parvoviridae Infections / metabolism
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Parvoviridae Infections / virology
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Serotyping
Substances
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Capsid Proteins
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Microtubule-Associated Proteins
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Dyneins