Recommended method for recovery of Toxocara and other geohelminth eggs from soil

Wiad Parazytol. 2005;51(1):21-2.

Abstract

The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H2O for washing the sample and next with the saturated sodium nitrate (NaNO3) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.

Publication types

  • Review

MeSH terms

  • Animals
  • Ecology / methods
  • Ovum
  • Soil / analysis*
  • Soil / parasitology*
  • Specimen Handling
  • Toxocara / isolation & purification*

Substances

  • Soil