Linkage mapping of highly informative DNA polymorphisms within the human interferon-alpha receptor gene on chromosome 21

Genomics. 1991 Nov;11(3):573-6. doi: 10.1016/0888-7543(91)90064-l.

Abstract

Two polymorphic loci within the interferon-alpha receptor (IFNAR) gene on human chromosome 21 have been identified and mapped by linkage analysis in 40 CEPH families. These markers are (1) a multiallelic RFLP with an observed heterozygosity of 0.72 and (2) a variable (AT3)n short sequence repeat at the poly(A) tail of an Alu sequence (AluVpA) with an observed heterozygosity of 0.83. This locus is close to D21S58 (theta = 0.02, zeta = 36.76) and D21S17 (theta = 0.02, Zeta = 21.76) with chromosomal band 21q22.1. Multipoint linkage analysis suggests the most likely locus order to be 21cen-D21S58-IFNAR-D21S17-21qter. Given its high heterozygosity, the IFNAR gene can be used as an index marker on human chromosome 21.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 21*
  • Female
  • Genetic Linkage / genetics*
  • Genetic Markers / genetics
  • Heterozygote
  • Humans
  • Interferon-alpha / metabolism*
  • Introns / genetics
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Polymorphism, Restriction Fragment Length*
  • Receptors, Immunologic / genetics*
  • Receptors, Interferon
  • Repetitive Sequences, Nucleic Acid / genetics

Substances

  • Genetic Markers
  • Interferon-alpha
  • Receptors, Immunologic
  • Receptors, Interferon