Abstract
Heparin oligosaccharides derived by nitrous acid depolymerization of heparin have been immobilized on amine-coated glass slides. The formation of a Schiff base creates heparin chips that are a suitable platform for the high-throughput analysis of carbohydrate-protein interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antithrombin III / chemistry
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Antithrombin III / metabolism
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Coated Materials, Biocompatible
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Fibroblast Growth Factor 1 / chemistry
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Fibroblast Growth Factor 1 / metabolism
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Fibroblast Growth Factor 2 / chemistry
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Fibroblast Growth Factor 2 / metabolism
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Heparin / chemistry*
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Humans
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Nitrous Acid / chemistry*
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Oligosaccharides / chemistry*
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Protein Array Analysis / instrumentation*
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Protein Array Analysis / methods
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Proteins / chemistry*
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Proteins / metabolism
Substances
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Coated Materials, Biocompatible
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Oligosaccharides
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Proteins
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Fibroblast Growth Factor 2
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Fibroblast Growth Factor 1
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Antithrombin III
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Heparin
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Nitrous Acid