Abstract
Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Base Sequence
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DNA Probes / genetics*
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Equipment Design
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Equipment Failure Analysis
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Expressed Sequence Tags*
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In Situ Hybridization, Fluorescence / instrumentation*
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In Situ Hybridization, Fluorescence / methods
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Molecular Probe Techniques / instrumentation*
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Molecular Sequence Data
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Reproducibility of Results
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Sensitivity and Specificity
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Sequence Alignment / instrumentation*
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Sequence Alignment / methods
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Sequence Analysis, DNA / instrumentation*
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Sequence Analysis, DNA / methods