Polymerase chain reaction (PCR) amplification of hypervariable genomic sequences

P Mandich; E Bellone; A Massari; F Ajmar

Polymerase chain reaction (PCR) amplification of hypervariable genomic sequences

Boll Soc Ital Biol Sper. 1991 Oct-Nov;67(10-11):907-13.

Authors

P Mandich¹, E Bellone, A Massari, F Ajmar

Affiliation

¹ Institute of Biology and Genetics (IBiG), University of Genoa.

PMID: 1687950

Abstract

This paper describes a rapid method for VNTRs (variable number of tandem repeats) typing using polymerase chain reaction (PCR). Three VNTRs (YNZ22, Apo B, MCT118) were amplified and alleles mendelian segregation was confirmed. We also demonstrate their applicability to paternity testing and forensic purposes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Apolipoproteins B / genetics
Base Sequence
Female
Genetic Variation*
Humans
Male
Molecular Sequence Data
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid*

Substances

Apolipoproteins B