Optimized protocols for the isolation of specific protein-binding peptides or peptoids from combinatorial libraries displayed on beads

Mol Biosyst. 2006 Jan;2(1):25-35. doi: 10.1039/b514349g. Epub 2005 Nov 28.

Abstract

Many methods have been published by which combinatorial libraries may be screened for compounds capable of manipulating the function(s) of a target protein. One of the simplest approaches is to identify compounds in a library that bind the protein of interest, since these binding events usually occur on functionally important surfaces of the protein. These protein-binding compounds could also be of utility as protein capture agents in the construction of protein-detecting microarrays or related analytical devices and as reagents for the affinity purification of proteins from complex mixtures. In this article, we provide optimized methods for screening libraries of molecules displayed on the beads on which they were synthesized. This is a particularly convenient format for library screening for laboratories with limited budgets and modest robotics capabilities.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chalcone
  • Combinatorial Chemistry Techniques / methods
  • Kinetics
  • Ligands
  • Models, Molecular
  • Myelin Basic Protein / chemistry
  • Peptide Library*
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Protein Binding

Substances

  • Ligands
  • Myelin Basic Protein
  • Peptide Library
  • Peptides
  • Chalcone