Abstract
The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.
MeSH terms
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Amino Acid Sequence
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Amino Acids / chemistry
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Buffers
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Fluorescent Dyes / analysis*
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Hydrogen-Ion Concentration
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Hydrophobic and Hydrophilic Interactions
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Models, Biological
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Proteins / chemistry
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Proteins / metabolism*
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Spectrometry, Fluorescence
Substances
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Amino Acids
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Buffers
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Fluorescent Dyes
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Proteins