Applications of polymerase chain reaction methods in genome mapping

Curr Opin Genet Dev. 1991 Jun;1(1):62-8. doi: 10.1016/0959-437x(91)80043-l.

Abstract

Important new polymerase chain reaction-based techniques have been developed to assist in genome analysis. Applications range from genetic and physical mapping of DNA to sequence analysis. The polymerase chain reaction has played a significant role in increasing the feasibility of many aspects of genome analysis and positional cloning.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Chromosome Mapping / methods*
  • Cloning, Molecular / methods
  • DNA Mutational Analysis
  • Genetic Markers
  • Genetic Vectors
  • Genome*
  • Genomic Library
  • Humans
  • Mice
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length
  • Repetitive Sequences, Nucleic Acid
  • Saccharomyces cerevisiae
  • Sequence Tagged Sites*

Substances

  • Genetic Markers