Abstract
High-affinity-binding sites for the vasoactive intestinal peptide (VIP) analogs peptide histidine/isoleucine-amide (PHI)/carboxyterminal methionine instead of isoleucine (PHM) are expressed in numerous tissues in the body but the nature of their receptors remains to be elucidated. The data presented indicate that PHI discriminated a high-affinity guanosine 5'-triphosphate (GTP)-insensitive-binding subtype that represented the totality of the PHI-binding sites in newborn rat tissues but was differentially expressed in adult animals. The GTP-insensitive PHI/PHM-binding sites were also observed in CHO cells over expressing the VPAC2 but not the VPAC1 VIP receptor.
MeSH terms
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Animals
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Binding Sites
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CHO Cells
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Cricetinae
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Guanosine Triphosphate / pharmacology*
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Liver / drug effects*
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Liver / metabolism*
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Peptide PHI / metabolism*
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Peptide PHI / pharmacology*
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Rats
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Receptors, Vasoactive Intestinal Peptide, Type II / genetics
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Receptors, Vasoactive Intestinal Peptide, Type II / metabolism
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Receptors, Vasoactive Intestinal Polypeptide, Type I / genetics
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Receptors, Vasoactive Intestinal Polypeptide, Type I / metabolism
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Recombinant Proteins / metabolism
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Sensitivity and Specificity
Substances
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Peptide PHI
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Receptors, Vasoactive Intestinal Peptide, Type II
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Receptors, Vasoactive Intestinal Polypeptide, Type I
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Recombinant Proteins
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Guanosine Triphosphate