Abstract
Objective:
To evaluate whether T cells expressing CCR5 and CXCR3 from multiple sclerosis (MS) patients are more resistant to apoptosis.
Methods:
Expression of CD69, TNF-R1, Fas, FasL, bcl-2, and bax was investigated in 41 MS patients and 12 healthy controls by flow cytometry in CD4+ and CD8+ T cells expressing CCR5 and CXCR3.
Results:
In MS patients, the percentage of CD69 was increased and Fas expression decreased in CD4+ CCR5+ T cells.
Interpretation:
The lower Fas expression in activated CD4+ CCR5+ T cells might contribute to disease pathogenesis by prolonging cell survival and favoring their migration into the CNS.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antigens, CD / immunology
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Antigens, CD / metabolism
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Antigens, Differentiation, T-Lymphocyte / immunology
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Antigens, Differentiation, T-Lymphocyte / metabolism
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Apoptosis / immunology*
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Autoimmunity / immunology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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Cell Survival / immunology
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Chemotaxis, Leukocyte / immunology
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Down-Regulation / immunology
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Fas Ligand Protein / immunology
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Fas Ligand Protein / metabolism
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Female
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Humans
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Immunity, Cellular / immunology
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Lectins, C-Type
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Male
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Middle Aged
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Multiple Sclerosis / immunology*
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Multiple Sclerosis / physiopathology
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Proto-Oncogene Proteins c-bcl-2 / immunology
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Receptors, CCR5 / biosynthesis
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Receptors, CCR5 / immunology*
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TNF Receptor-Associated Death Domain Protein / immunology
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TNF Receptor-Associated Death Domain Protein / metabolism
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bcl-2-Associated X Protein / immunology
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bcl-2-Associated X Protein / metabolism
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fas Receptor / immunology*
Substances
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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CD69 antigen
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Fas Ligand Protein
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Lectins, C-Type
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Proto-Oncogene Proteins c-bcl-2
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Receptors, CCR5
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TNF Receptor-Associated Death Domain Protein
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Tradd protein, mouse
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bcl-2-Associated X Protein
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fas Receptor