Use of synthetic oligonucleotide DNA probes for the identification of Bacteroides gingivalis

J Clin Microbiol. 1990 Feb;28(2):324-7. doi: 10.1128/jcm.28.2.324-327.1990.

Abstract

Six different oligonucleotide probes complementary to the hypervariable regions of 16S rRNA of Bacteroides gingivalis were tested for specificity and sensitivity against 77 field strains of B. gingivalis and 105 strains of 12 other Bacteroides species. The data demonstrated that these probes were very specific (range, 0.85 to 1.00) and sensitive (1.00). Some limited cross-reactions with other Bacteroides species were observed. Four of these probes should be useful for rapid detection and identification of B. gingivalis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteroides / classification
  • Bacteroides / genetics
  • Bacteroides / isolation & purification*
  • DNA Probes*
  • Humans
  • Nucleic Acid Hybridization
  • Periodontal Diseases / microbiology
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Species Specificity

Substances

  • DNA Probes
  • RNA, Bacterial
  • RNA, Ribosomal, 16S