Objective: To survey the role of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in early embryo development, we study the HA-1 gene expressing in villous tissue after embryo implantation.
Methods: Fifty villous tissue samples from pregnant 6 w to 10 w were divided into five groups and each group included ten samples. The HAI-1 gene and protein expressing in the villous tissue were detected by RT-PCR and immunohistochemistry.
Results: The expression of HAI-1 had a gradual increase with the pregnancy weeks added. The HAl-1 gene expression was very low at pregnant 6 w(A(260/280) 0.3142), From pregnant 7 w to 9 w, the expression was increased gradually (A(260/280) 0.3476 in 7 w and 0.3910 in 8 w) and reached the pinnacle in 9 w (A(260/280) 0.4876). And then the pinnacle value lasted until 10 w (A(260/280) 0.4909). The proportion of HAI-1 mRNA/beta-actin mRNA between 9 w and 10 w was not significantly different (P > or = 0.05). But among the other 3 groups, the proportion was significantly different (P < 0.01). The proportion of 9 w and 10 w was significantly different from the other 3 groups (P < 0.01). The results of HAI-1 protein expression showed the similar conclusion by immunohistochemical analysis.
Conclusion: The HAI-1 gene may play an important role in early embryo development in human.