Abstract
Inducible cell adhesion molecule 110 (INCAM-110) is a 110-kD glycoprotein expressed on cytokine-activated human vascular endothelial cells. mAb blocking studies indicate that INCAM-110 and intercellular adhesion molecule 1 (ICAM-1) independently support the adhesion of lymphocytes to activated human umbilical vein endothelial cell monolayers. Anti-CD11a/CD18 antibodies with anti-INCAM-110 mAb E1/6 produce greater inhibition of lymphocyte adhesion than either reagent alone, suggesting that INCAM-110 and LFA-1 are not an obligate receptor-ligand pair. Blood monocytes, but not polymorphonuclear leukocytes, also appear to bind endothelial INCAM-110. Endothelial expression of INCAM-110 is upregulated at sites of inflammation, suggesting a role in the recruitment of mononuclear leukocytes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Antigens, CD / immunology*
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Antigens, Differentiation / immunology*
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CD11 Antigens
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CD18 Antigens
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Cell Adhesion Molecules / biosynthesis
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / physiology*
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Cell Adhesion*
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Cell Line
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Endothelium, Vascular / immunology
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Endothelium, Vascular / physiology*
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Female
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Flow Cytometry
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Humans
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Hypersensitivity, Delayed
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Immunoenzyme Techniques
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Inflammation
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Lymphocytes / immunology*
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Pregnancy
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Receptors, Leukocyte-Adhesion / immunology*
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Reference Values
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Skin / immunology
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Umbilical Veins
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Vascular Cell Adhesion Molecule-1
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation
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CD11 Antigens
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CD18 Antigens
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Cell Adhesion Molecules
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Receptors, Leukocyte-Adhesion
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Vascular Cell Adhesion Molecule-1