Abstract
We describe an immunoperoxidase technique for distinguishing melanocytes and melanophages in paraffin-embedded tissue. Two primary antibodies were used: anti-S100 and HAM-56, a monoclonal antibody directed against macrophages. In a variety of pigmented lesions, HAM-56 labelled melanophages while S100 labelled cells of melanocytic lineage.
MeSH terms
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Amyloidosis / pathology
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Antibodies
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Antibodies, Monoclonal
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Histiocytes / pathology*
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Humans
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Immunoenzyme Techniques*
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Melanins
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Melanocytes / pathology*
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Melanoma / pathology*
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Nevus / pathology*
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Paraffin
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Phagocytosis
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S100 Proteins / analysis
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Skin Neoplasms / pathology*
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Staining and Labeling
Substances
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Antibodies
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Antibodies, Monoclonal
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Melanins
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S100 Proteins
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Paraffin