Abstract
Peptide nucleic acids (PNA) are very promising antisense agents, but their in vivo application is often hampered by their low bioavailability, mainly due to their limited uptake through cellular and nuclear membranes. However, PNA chemical synthesis easily allows modification with functional structures able to improve the intrinsically low permeability and great interest is arising in finding specific and efficient delivery protocols. Polymeric core-shell microspheres with anionic functional groups on the surface were tested for their ability to reversibly bind lysine modified PNA sequences, whose antisense activity against COX-2 mRNA was already demonstrated in murine macrophages.
MeSH terms
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Acrylic Resins
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Animals
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Antisense Elements (Genetics)*
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Biological Availability
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Cyclooxygenase 2 / biosynthesis
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Cyclooxygenase 2 / genetics
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Excipients
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Hydrogen-Ion Concentration
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In Vitro Techniques
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Microscopy, Confocal
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Microscopy, Electron, Scanning
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Microspheres
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Particle Size
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Peptide Nucleic Acids / administration & dosage*
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Peptide Nucleic Acids / pharmacology*
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Peptide Nucleic Acids / toxicity
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Polymethyl Methacrylate
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Thermodynamics
Substances
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Acrylic Resins
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Antisense Elements (Genetics)
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Eudragit L100-55
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Excipients
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Lipopolysaccharides
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Peptide Nucleic Acids
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RNA, Messenger
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Polymethyl Methacrylate
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Cyclooxygenase 2