Differential cellular catabolism of 111In, 90Y and 125I radiolabeled T101 anti-CD5 monoclonal antibody

Y Naruki; J A Carrasquillo; J C Reynolds; P J Maloney; J M Frincke; R D Neumann; S M Larson

doi:10.1016/0883-2897(90)90148-t

Differential cellular catabolism of 111In, 90Y and 125I radiolabeled T101 anti-CD5 monoclonal antibody

Int J Rad Appl Instrum B. 1990;17(2):201-7. doi: 10.1016/0883-2897(90)90148-t.

Authors

Y Naruki¹, J A Carrasquillo, J C Reynolds, P J Maloney, J M Frincke, R D Neumann, S M Larson

Affiliation

¹ Department of Nuclear Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892.

PMID: 1692819
DOI: 10.1016/0883-2897(90)90148-t

Abstract

We evaluated the differences in catabolism of 125I, 111In and 90Y-T101 monoclonal antibody (anti-CD5) by peripheral blood mononuclear cells (PBMNC), HUT 102, CCRF-CEM and MOLT-4 cells. All cells showed higher retention of 111In than of 125I. PBMNC showed similar retention of 90Y and 111In. The release of 125I was reduced by using metabolic inhibitors, F(ab')2 of T101, pure lymphocytes or incubating at 4 degrees C. Our findings suggest differences in the intracellular catabolism of the radionuclides.

Publication types

Comparative Study

MeSH terms

Animals
Antibodies, Monoclonal / metabolism*
Antigens, Differentiation / immunology*
CD5 Antigens
Cell Line
Humans
In Vitro Techniques
Indium Radioisotopes*
Iodine Radioisotopes*
Leukocytes, Mononuclear / metabolism*
Mice
Tumor Cells, Cultured / metabolism*
Yttrium Radioisotopes*

Substances

Antibodies, Monoclonal
Antigens, Differentiation
CD5 Antigens
Indium Radioisotopes
Iodine Radioisotopes
Yttrium Radioisotopes