Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

Christian B F Andersen; Lionel Ballut; Jesper S Johansen; Hala Chamieh; Klaus H Nielsen; Cristiano L P Oliveira; Jan Skov Pedersen; Bertrand Séraphin; Hervé Le Hir; Gregers Rom Andersen

doi:10.1126/science.1131981

Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

Science. 2006 Sep 29;313(5795):1968-72. doi: 10.1126/science.1131981. Epub 2006 Aug 24.

Authors

Christian B F Andersen¹, Lionel Ballut, Jesper S Johansen, Hala Chamieh, Klaus H Nielsen, Cristiano L P Oliveira, Jan Skov Pedersen, Bertrand Séraphin, Hervé Le Hir, Gregers Rom Andersen

Affiliation

¹ Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark.

PMID: 16931718
DOI: 10.1126/science.1131981

Abstract

In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / analogs & derivatives
Adenosine Triphosphate / metabolism
Adenylyl Imidodiphosphate / metabolism
Amino Acid Motifs
Amino Acid Sequence
Animals
Crystallography, X-Ray
DEAD-box RNA Helicases
Dimerization
Drosophila Proteins / chemistry
Drosophila Proteins / metabolism
Eukaryotic Initiation Factor-4A / chemistry*
Eukaryotic Initiation Factor-4A / metabolism
Exons*
Humans
Hydrogen Bonding
Hydrolysis
Models, Molecular
Molecular Sequence Data
Mutation
Neoplasm Proteins / chemistry*
Neoplasm Proteins / metabolism
Nuclear Proteins / chemistry*
Nuclear Proteins / metabolism
Nucleic Acid Conformation
Poly U / chemistry*
Poly U / metabolism
Protein Conformation
Protein Structure, Secondary
Protein Structure, Tertiary
RNA Helicases / chemistry
RNA Helicases / metabolism
RNA, Messenger / chemistry*
RNA, Messenger / metabolism
RNA-Binding Proteins / chemistry*
RNA-Binding Proteins / metabolism

Substances

CASC3 protein, human
Drosophila Proteins
MAGOH protein, human
Neoplasm Proteins
Nuclear Proteins
RBM8A protein, human
RNA, Messenger
RNA-Binding Proteins
Adenylyl Imidodiphosphate
Poly U
Adenosine Triphosphate
Eukaryotic Initiation Factor-4A
vas protein, Drosophila
DEAD-box RNA Helicases
RNA Helicases

Associated data

PDB/2HXY
PDB/2HYI