Mode of transposition and expression of 16S rRNA methyltransferase gene rmtC accompanied by ISEcp1

Jun-ichi Wachino; Kunikazu Yamane; Kouji Kimura; Naohiro Shibata; Satowa Suzuki; Yasuyoshi Ike; Yoshichika Arakawa

doi:10.1128/AAC.00550-06

Mode of transposition and expression of 16S rRNA methyltransferase gene rmtC accompanied by ISEcp1

Antimicrob Agents Chemother. 2006 Sep;50(9):3212-5. doi: 10.1128/AAC.00550-06.

Authors

Jun-ichi Wachino¹, Kunikazu Yamane, Kouji Kimura, Naohiro Shibata, Satowa Suzuki, Yasuyoshi Ike, Yoshichika Arakawa

Affiliation

¹ Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.

Abstract

A newly identified 16S rRNA methyltransferase gene, rmtC, was accompanied by an ISEcp1 element at its 5' end. This ISEcp1 element, which contained a transposase gene, tnpA, provided a promoter activity for expression of the adjacent rmtC; and this structure enabled the rmtC gene to be transposed onto another plasmid in Escherichia coli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA Transposable Elements / genetics*
Escherichia coli / enzymology
Escherichia coli / genetics
Methyltransferases / biosynthesis
Methyltransferases / genetics*
Molecular Sequence Data
Plasmids / genetics
Proteus mirabilis / isolation & purification
RNA, Ribosomal, 16S / biosynthesis*
RNA, Ribosomal, 16S / genetics

Substances

DNA Transposable Elements
RNA, Ribosomal, 16S
Methyltransferases

Associated data

GENBANK/AB194779