[Construction and characterization of combinatorial naive phage library of human scFvs]

T A Batanova; A B Ulitin; V V Morozova; A G Laman; E V Zhirakovskaia; V V Dubrovskaia; F A Brovko; N V Tikunova

[Construction and characterization of combinatorial naive phage library of human scFvs]

Mol Gen Mikrobiol Virusol. 2006:(3):35-41.

[Article in Russian]

Authors

T A Batanova, A B Ulitin, V V Morozova, A G Laman, E V Zhirakovskaia, V V Dubrovskaia, F A Brovko, N V Tikunova

PMID: 16941846

Abstract

A combinatorial phage display library of human single-chain antibody fragments (scFv) was constructed on the basis of variable domains of heavy (Vh) and light (VI) genes cloned from the lymphocytes of six healthy donors. The size of the library was 2? 10(8) independent clones. Single-chain antibodies against recombinant human TNF?, vaccinia virus and virus-like particles formed by core protein of hepatitis B virus were selected from the library. Unique scFv sequences were identified using the HaeIII fingerprinting. The specificity of the selected clones was proved by the Western-blot analysis.

Publication types

English Abstract

MeSH terms

Antibody Specificity
Bacteriophage M13 / metabolism
Hepatitis B Core Antigens / immunology
Humans
Immunoglobulin Fragments / immunology*
Immunoglobulin Fragments / metabolism*
Immunoglobulin Variable Region / immunology*
Immunoglobulin Variable Region / metabolism*
Leukocytes, Mononuclear
Peptide Library*
RNA, Messenger
Recombinant Proteins / biosynthesis
Recombinant Proteins / immunology
Tissue Donors
Tumor Necrosis Factor-alpha / immunology
Vaccinia virus / immunology

Substances

Hepatitis B Core Antigens
Immunoglobulin Fragments
Immunoglobulin Variable Region
Peptide Library
RNA, Messenger
Recombinant Proteins
Tumor Necrosis Factor-alpha