Inhibition of human immunodeficiency virus envelope glycoprotein- mediated single cell lysis by low-molecular-weight antagonists of viral entry

J Virol. 2007 Jan;81(2):532-8. doi: 10.1128/JVI.01079-06. Epub 2006 Aug 30.

Abstract

The coexpression of human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins and receptors leads to the lysis of single cells by a process that is dependent upon membrane fusion. This cell lysis was inhibited by low-molecular-weight compounds that interfere with receptor binding or with receptor-induced conformational transitions in the envelope glycoproteins. A peptide, T20, potently inhibited cell-cell fusion but had no effect on single cell lysis mediated by the HIV-1 envelope glycoproteins. Thus, critical events in the lysis of single cells by the HIV-1 envelope glycoproteins occur in intracellular compartments accessible only to small inhibitory compounds.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cytopathogenic Effect, Viral / drug effects
  • Dogs
  • Enfuvirtide
  • Ethers / pharmacology*
  • Gene Products, env / metabolism*
  • HIV Envelope Protein gp41 / pharmacology
  • HIV-1 / drug effects*
  • HIV-1 / pathogenicity*
  • Humans
  • Hydrocarbons, Fluorinated / pharmacology*
  • Membrane Fusion / drug effects*
  • Molecular Weight
  • Peptide Fragments / pharmacology
  • Piperazines / pharmacology*

Substances

  • BMS 806
  • Ethers
  • Gene Products, env
  • HIV Envelope Protein gp41
  • Hydrocarbons, Fluorinated
  • Peptide Fragments
  • Piperazines
  • Enfuvirtide
  • fluoromethyl 2,2-difluoro-1-(trifluoromethyl)vinyl ether