Comparison of MS/MS methods for protein identification from 2D-PAGE

Giorgio Arrigoni; Celine Fernandez; Cecilia Holm; Michaela Scigelova; Peter James

doi:10.1021/pr0601281

Comparison of MS/MS methods for protein identification from 2D-PAGE

J Proteome Res. 2006 Sep;5(9):2294-300. doi: 10.1021/pr0601281.

Authors

Giorgio Arrigoni¹, Celine Fernandez, Cecilia Holm, Michaela Scigelova, Peter James

Affiliation

¹ Department of Protein Technology, Lund University, Sweden.

PMID: 16944941
DOI: 10.1021/pr0601281

Abstract

We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Computational Biology
Electrophoresis, Gel, Two-Dimensional / methods*
Proteins / analysis*
Proteins / isolation & purification
Proteomics / methods*
Spectrometry, Mass, Electrospray Ionization / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Proteins