Abstract
We have mapped a linear epitope recognized by the virus-neutralizing monoclonal antibody 6-15C4 within the primary sequence of the G protein from the Evelyn-Rokitnicki-Abelseth strain of rabies virus. This was accomplished by using fragments of the rabies virus G protein and deduced amino acid sequences of neutralization-resistant variant rabies viruses. The monoclonal antibody 6-15C4 specifically recognized a synthetic peptide (peptide G5-24) which resembles the 6-15C4 epitope in structure. In addition, a tandem peptide constructed from the G5-24 peptide and a dominant TH cell epitope of the rabies virus N protein induced protective immunity against lethal rabies virus challenge infection in mice.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / immunology
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Antibodies, Viral / immunology
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Antibody Formation
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Antigens, Viral*
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Base Sequence
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Epitopes / analysis
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Epitopes / genetics*
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Genes, Viral
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Genetic Variation
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Glycoproteins / genetics*
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Glycoproteins / immunology
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Glycoproteins / isolation & purification
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Lymphocyte Activation
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Molecular Sequence Data
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Neutralization Tests
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Peptides / chemical synthesis
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RNA, Viral / genetics
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Rabies virus / genetics*
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Rabies virus / immunology
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T-Lymphocytes, Helper-Inducer / immunology
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Vaccines, Synthetic
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Viral Envelope Proteins*
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Viral Proteins / genetics*
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Viral Proteins / immunology
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Viral Proteins / isolation & purification
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Viral Structural Proteins / genetics
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antigens, Viral
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Epitopes
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Glycoproteins
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Peptides
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RNA, Viral
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Proteins
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Viral Structural Proteins
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glycoprotein G, Rabies virus