Abstract
Neural stem cells differentiate from embryonic stem cells via formation of neural stem spheres under free-floating conditions in astrocyte-conditioned medium. Subsequent culture of neural stem spheres on an adhesive substrate with fibroblast growth factor-2 promotes the migration of neural stem cells onto the substrate, resulting in an increase in the number of cells. These embryonic stem cell-derived neural stem cells can be differentiated almost exclusively into astrocytes by withdrawing fibroblast growth factor-2 from the medium without any additional instructions.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Astrocytes / chemistry*
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Astrocytes / physiology
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Cell Count
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cell Movement / drug effects
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Cell Movement / physiology
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Cells, Cultured
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Culture Media, Conditioned / pharmacology
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Embryo, Mammalian
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Fibroblast Growth Factor 2 / pharmacology
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Gene Expression / drug effects
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Glial Fibrillary Acidic Protein / metabolism
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Immunohistochemistry / methods
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Mice
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neurons / drug effects
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Neurons / physiology*
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Stem Cells / drug effects
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Stem Cells / physiology*
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Time Factors
Substances
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Culture Media, Conditioned
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Glial Fibrillary Acidic Protein
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Nerve Tissue Proteins
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RNA, Messenger
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Fibroblast Growth Factor 2