[The ts phenotype of reisolates from children inoculated with live cold-adapted influenza vaccine type A]

Vopr Virusol. 1990 Mar-Apr;35(2):101-5.
[Article in Russian]

Abstract

Using mutants of fowl plague virus (FRV) which have single temperature-sensitive (ts) mutations in some genes, an analysis was carried out on reisolates from children of 3-6 years, vaccinated with a monovaccine from recombinant strains of influenza type A virus. The recombinants were obtained by crossing of current epidemic strains of subtypes A (HINI) and a (H3N2) with the cold-adapted (XA) ts-donor of attenuation A/Leningrad/134/47/57 (H2N2) from which they, as a rule, inherited 5 ts-mutations in genes 1 (PB2), 2 (PB1), 5 (NP), 7 (M), and 8 (NS). All the reisolates were shown to retain the ts-phenotype. However, in the recombination test some reisolates (most frequently those isolated at late periods of vaccination infection) no ts-mutations could be found in 1-3 genes coding for proteins of the polymerase complex, less frequently for NP and NS proteins but not for M proteins.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Antigens, Viral / analysis
  • Antigens, Viral / genetics
  • Child
  • Child, Preschool
  • Cold Temperature
  • Epitopes / analysis
  • Epitopes / genetics
  • Genes, Viral / genetics
  • Humans
  • Influenza A virus / genetics
  • Influenza A virus / immunology
  • Influenza A virus / isolation & purification*
  • Influenza Vaccines / administration & dosage
  • Influenza Vaccines / genetics*
  • Influenza Vaccines / immunology
  • Mutation
  • Phenotype
  • Recombination, Genetic / genetics
  • Serial Passage
  • Temperature*
  • Time Factors

Substances

  • Antigens, Viral
  • Epitopes
  • Influenza Vaccines